Introduction: There are many graft options available for anterior cruciate ligament reconstruction. Because of the shorter length and unpredictability of graft size the hamstring graft the peroneus longus tendon could be a good option for anterior cruciate ligament reconstruction. Peroneus longus tendon provides thicker, longer and stiff graft and it had promising results in anterior cruciate ligament reconstruction. Peroneus graft harvesting doesn't affect ankle functions. Aim & objectives: To evaluate the functional outcome of ACL reconstruction using peroneus longus and semitendinosus graft and effect on ankle morbidity and ankle function. Materials & methods: This was a retrospective study that included 50 patients of anterior cruciate ligament injury treated with anterior cruciate ligament reconstruction with peroneus longus tendon graft with semitendinosus graft. Results were assessed by physical examinations. At follow-up Lysholm scores were calculated. For ankle function American Orthopaedic Foot and Ankle Society (AOFAS) score and The Foot and Ankle Disability Index (FADI) was evaluated. Ankle muscle power assessment was done as per Medical Research Council (MRC) grades. Results: This study includes 50. The average thickness of semitendinosus and peroneus longus tendon coupled grafts was 9.3 mm. The average Lysholm score was 93.5 (range 80-100). Among all patients 74% patients had an excellent Lysholm score, 20% had a good score and 6% had a fair score. The average FADI score was 134.8 (range 120-136). The average AOFAS score was 97.7 (range 95-100). Conclusion: Peroneus longus tendon graft had promising results in ACL reconstruction with advantages in form of easy to harvest, thicker and stiff graft, longer graft length, no muscle wasting with no effect on ankle plantarflexion, eversion and on ankle stability with good functional outcome so it can be coupled with hamstring graft for better stable ACL reconstruction.