Background with Purpose: The aim of the study undertaken was to assess the effect of pronated foot on Quadriceps angle, lumbar lordosis index and thoracic kyphosis index. Methodology: The study was cross sectional study conducted after screening for inclusion and exclusion criteria on 58 subjects. The subjects were divided into 2 groups based on navicular drop value. Group A - Normal feet group (29), consisted of 5 males and 24 females with the mean age of 22.03 ± 2.784 years. Group B - Pronated feet group (29), consisted of 6 males and 23 females with the mean age of 21.75 ± 2.016 years.The participants in both the groups were assessed for navicular drop value using broady’s navicular drop test. Outcome measures included was Q angle, lumbar lordosis index and thoracic kyphosis index which were measured in erect standing position. Results: The results showed that there was significant difference observed in the Q angle (right and left side), lumbar lordosis index and thoracic kyphosis index between 2 groups (p value- <0.001). Conclusion: From the present study it can be concluded that pronated feet alter the lower extremity and spinal alignment. Therefore, considering the effect of distal segment alterations on proximal kinetic chain is essential for the precise assessment and treatment of musculoskeletal system.