Background: Myofascial Trigger Points is a hyperirritable spot in a taut band of skeletal muscle that is the main reason for pain and dysfunction in the upper limb. Trapezius muscle has three different portions: the upper trapezius that originates from the superior nuchal line to the ligamentum nuchae above C7 spinal process and attaches the posterior border of the distal third of the clavicle. There is evidence for a relationship between highly repetitive work and neck and neck/shoulder musculoskeletal disorders. Repetitive work for the neck as work activities which involve sustained arm or hand movements can affect the neck/shoulder musculature and generate loads on the neck/shoulder area. Objectives: We hypothesis that fatigue can alter cervical spine range of motion because of transferring loads to the passive connective tissues and also increasing muscle activity. Methods: This is a Randomized controlled trial study. Thirty-six volunteers were recruited to determine EMG signal slops in submaximal trial of the trapezius muscle during shoulder shrug. Results: The behavior of the upper trapezius muscle was seen to be similar when assessment was based. Improvements in pain and left-lateral flexion were found to be greater in the upper trapezius muscle-Group compared to the control group (p < 0.05). Fatigue applied to the upper trapezius muscle reduce pain and disability, and increase ROM and disability in individuals with myofascial pain syndrome. Conclusions: This treatment may therefore be considered for use as an alternative method in treating trigger point.